ABSTRACT
<p><b>OBJECTIVE</b>To evaluate whether or not administration of folic acid and resveratrol have preventive effects on cleft palate formation as well as the comparison of the two drugs' s effects.</p><p><b>METHODS</b>Pregnant mice were randomly divided into 9 groups, with 8 mice in each group. The TCDD group mice were dosed with TCDD 28 microg/kg body weight on gestation day 10 (GD 10) animals in folic acid group were respectively dosed with folic acid 15, 10, 5 mg/kg and TCDD 28 microg/kg; resveratrol treated mice were divided into 3 groups: resveratrol 50 mg/kg were orally administered for 6 consecutive days, from gestational day GD 8 to GD13 in resveratrol (GD8-13 ) group; resveratrol 50 mg/kg were orally administered for 6 consecutive days, from gestational day GD 8 to GD13, followed hy an oral administered with TCDD on GD10 in resveratrol (GD8-13) + TCDD group; resveratrol 50mg/kg and TCDD 28 microg/kg were used by gavage administration at GD10 in resveratrol (GD10) + TCDD group. Control mice were treated with the same volume of water for 6 consecutive days from GD8 to GD13 and were given a single dose of corn oil on GD10. The pregnant mice weight and embryos, the number of live, cleft palate, dead and resorption fetal mice were recorded on GD 17.5. The coronal sections of the fetal mice heads were prepared at GD 17.5 and observed by microscopy.</p><p><b>RESULTS</b>Total frequency of clefts was 92.86% in TCDD group, 84.00% (15 mg), 73.08% (10 mg), 84.00% (5 mg) in folic acid + TCDD groups, 0% in resveratrol (GD10) group, 74.51% (GD10), 57.78% (GD8-13) in resveratrol + TCDD groups. The frequency of cleft was 0% in the control group. Compared with the control and the TCDD groups, there were significant differences in the number of live, dead and resorption fetal mice in TCCD + resveratrol (GD8-13) group (P < 0.05). No significant differences in embryonic weight, live fetuses weight, the number of live, dead and resorption fetal mice were found in the other groups (P > 0.05).</p><p><b>CONCLUSION</b>Test dose of folic acid and resveratrol both had certain antagonistic effect on cleft palate in mice induced by TCDD, with folic acid 10 mg/kg, resveratrol 50 mg/kg GD8-13 doses having stronger antagonistic action. Effects of both the two drugs have no significant difference, but resveratrol (50 mg/kg, GD8-13) significantly affects the fetal mice's growth and development under TCDD exposure in utero.</p>
Subject(s)
Animals , Female , Humans , Mice , Pregnancy , Abnormalities, Drug-Induced , Cleft Palate , Fetus , Folic Acid , Pharmacology , Mice, Inbred C57BL , Polychlorinated Dibenzodioxins , Random Allocation , Stilbenes , Pharmacology , TeratogensABSTRACT
<p><b>OBJECTIVE</b>The aim of the present study was to investigate the difference of intolerance to food between southern and northern middle-aged Chinese, and furthermore analyze its association with eating habits in both study population.</p><p><b>METHODS</b>ELISA was applied to determine the serum concentrations of specific IgG of 14 food anaphylactogen in 1568 healthy subjects from totally 9 districts in both southern and northern China. Life style questionnaire was also applied to investigate the daily intake of six categorizes of food associated with food intolerance.</p><p><b>RESULTS</b>45.8% of all subjects were found to be intolerant to certain food. 62.3% of subjects from southern China and 40.4% of subjects from northern China were found to be intolerant to certain food, the difference between southern and northern Chinese was statistically significant. Top three foods intolerant by southern Chinese were crab, egg, and cold fish, while top three food intolerant by northern Chinese were egg, crab, and milk. The differences of intolerance to crab, cold fish, soy bean, rice, and tomato between southern and northern Chinese were statistically significant. Investigation on eating habits revealed that cereals and fish were the major food consumed by subjects in our study. There was no certain association between food intolerance and eating habits.</p><p><b>CONCLUSION</b>Considering that there are differences between southern and northern Chinese, southern and northern Chinese should pay attention to their daily food in order to avoid food allergy.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , China , Epidemiology , Feeding Behavior , Food Hypersensitivity , Epidemiology , Metabolic Diseases , Epidemiology , Surveys and QuestionnairesABSTRACT
<p><b>OBJECTIVE</b>To investigate the effective clinical diagnosis and treatment for allergic rhinitis (AR) by examining the allergens and dietary habits of the patients.</p><p><b>METHODS</b>A total of 347 patients from Lanzhou area were sampled between April 2010 and March 2012. Free point skin prick test were performed to analyze the allergens. SPSS 17.0 software was used to analyze the data.</p><p><b>RESULTS</b>Among 347 patients, 266 (76.7%) were found to be positive in the skin test. Major aeroallergens were Artemisia pollen (38.0%, 132 cases), house dust mite (35.4%, 123 cases), and Dermatophagoides farinae (30.3%, 105 cases).</p><p><b>CONCLUSIONS</b>The major aeroallergens in Lanzhou with AR were artemisia pollen and mite.</p>
Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Male , Middle Aged , Young Adult , Allergens , Allergy and Immunology , China , Epidemiology , Rhinitis, Allergic , Rhinitis, Allergic, Perennial , Diagnosis , Epidemiology , Allergy and Immunology , Skin TestsABSTRACT
<p><b>OBJECTIVE</b>To study methylation of Id4 gene and demethylation effect of arsenic trioxide (ATO) in Raji cells.</p><p><b>METHODS</b>Human Burkitt's Raji lymphoma cells were cultared and treated with ATO at different concentrations and different time points. Methylated degree of Id4 gene was detected by methylation specificity polymerase chain reaction (MS-PCR), Id4 mRNA expression in Raji cell by reverse transcription polymerase chain reaction (RT-PCR), the growth of cell by MTT assay, and cell apoptosis and cycle distribution by Flow Cytometry (FCM).</p><p><b>RESULTS</b>(1) The Id4 gene exhaustive methylation in control group, and hypermethylation in experimental group were reversed by ATO in a dose-dependent manner. (2) Id4 mRNA expression in Raji cells treated with ATO for 48 h increased gradually with ATO concentration increasing in experimental group. (3) Raji cell growth inhibited rates after different concentrations of ATO treatment for 24, 48, 72 h were 12.15% ∼ 92.17% in the experimental group (P < 0.05). (4) Apoptosis peak emerged after ATO treatment for 48 hours in experimental group, while a much lower apoptosis in control group. (5) After ATO treatment for 48 h in experiment group, the cells were arrested at G(0)/G(1) phase in a dose-dependent manner.</p><p><b>CONCLUSION</b>Id4 gene presents exhaustive methylation in Raji cells. ATO can reverse the hypermethylation of Id4 gene and recover the expression of Id4 mRNA. Hypermethylation of Id4 gene is one of the reasons of Raji cells malignant proliferations.</p>
Subject(s)
Humans , Apoptosis , Burkitt Lymphoma , Genetics , Cell Line, Tumor , DNA Methylation , RNA, Messenger , GeneticsABSTRACT
<p><b>OBJECTIVES</b>To unravel the molecular mechanism of proliferation inhibition induced by transfection of pemt2-cDNA into rat CBRH-7919 hepatoma cells.</p><p><b>METHODS</b>We started with the highly expressed PEMT2 clone. Cell culture and Western blotting techniques were used to examine the expression of cyclinD1/CDK4, cyclinE/CDK2, phospho-Rb, caspase-3, c-jun and caveolins.</p><p><b>RESULTS</b>Our results showed that CDK4, CDK2, phospho-Rb and c-jun were down regulated in the pemt2 highly expressed cell clone. The high expression clone of pemt2-transfected cells also showed over expression of caspase-3.</p><p><b>CONCLUSION</b>The reductions of proliferation and apoptosis of pemt2 transfected cells could be related to the G1 phase arrest induced by down-regulation of the cell cycle-associated proteins.</p>
Subject(s)
Animals , Rats , Apoptosis , Physiology , Caspase 3 , Genetics , Cyclin-Dependent Kinase 2 , Genetics , Cyclin-Dependent Kinase 4 , Genetics , Cyclin-Dependent Kinases , Genetics , Down-Regulation , Liver Neoplasms, Experimental , Genetics , Metabolism , Phosphatidylethanolamine N-Methyltransferase , Genetics , Metabolism , Transfection , Tumor Cells, CulturedABSTRACT
<p><b>AIM</b>To study the chemical structure of SC3, an acidic polysaccharide from Salvia chinesis.</p><p><b>METHODS</b>Based on chemical (including sugar composition analysis, methylation analysis, uronic acid reduction and partial acid hydrolysis) and spectral analysis (IR, NMR, ESI-MS), the structural characterization of SC3 was investigated.</p><p><b>RESULTS</b>SC3 was composed Rha, Ara, Gal and GalA, with its mean molecular weight of 7.7 x 10(4). By means of methylation analysis, partial acid hydrolysis, NMR and ESI-MS spectrum, the linkages and sequence information of SC3 were obtained.</p><p><b>CONCLUSION</b>SC3 is an complicated acidic polysaccharide, obtained for the first time from the plant.</p>
Subject(s)
Arabinose , Chemistry , Galactose , Chemistry , Molecular Structure , Molecular Weight , Plants, Medicinal , Chemistry , Polysaccharides , Chemistry , Rhamnose , Chemistry , Salvia , ChemistryABSTRACT
The present study demonstrated that bone marrow cells from diabetic rats were able to form cell clusters expressing insulin,C-peptide,glucagon,somatostatin and islet amyloid .polypeptide,and other genes associated with development and function of islets such as glucose transporter-2,glucokinase,glucagon-like peptide-1 receptor,PDX-1,Ngn3,NeuroDl,Pax-6 and NKX2.2 genes.These islet-like cells might be derived from adult stem cells in bone marrow.